Functional Peptides from One-bead One-compound High-throughput Screening Technique

摘要

Combinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides.One-bead one-compound(OBOC)high-throughput screening technique offers a lot of structurally diverse peptides to be rapidly synthesized and screened for binding to a target of interest.The OBOC peptide library screening involves three main steps:library construction,positive beads separation,and peptide sequencing.This review mainly summarizes some special technique tips during functional peptide screening and potential future directions of the OBOC high-throughput screening technique.