Considering the research on classical genetics of photoperiod(therm) sensitive genic male sterile rice, it is important to select the sterile lines and their segregating population controlled by one pair of gene in mapping and isolating sterile genes. It is discussed the advantages, disadvantages and the reasons leading to various mapping results of chromosome location of sterile genes through gene marker, isozyme marker and DNA marker techniques. In comparison to isolation of photo(thermo) sensitive sterile genes via various plant gene cloning techniques, it was concluded that map based cloning was acceptable, but it is still difficult to locate the loci of sterile genes within 1cM. On the other hand, “sensitivity to environment”, an important characteristic of sterile lines can be fully utilized by DD PCR and (or) RDA techniques. Therefore, these two techniques were considered as the effective ways to isolate sterile genes.
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机译:Analysis of GDSL lipase (GLIP) family genes in rice (Oryza sativa) Yunyun Jiang, Rongjun Chen, Jiali Dong, Zhengjun Xu, Xiaoling Gao Rice Research Institute of Sichuan Agricultural University, Chengdu, Sichuan 611130, China Abstract
机译:Analysis of GDSL lipase (GLIP) family genes in rice (Oryza sativa) Yunyun Jiang, Rongjun Chen, Jiali Dong, Zhengjun Xu, Xiaoling Gao Rice Research Institute of Sichuan Agricultural University, Chengdu, Sichuan 611130, China Abstract