两株不同地域的家蚕微孢子虫分离株的细胞侵染力比较及遗传多样性分析

摘要

家蚕微孢子虫(Nosema bombycis)是蚕业生产上一种毁灭性病害--微粒子病的病原体.文章将安徽和重庆两地域来源的病原性家蚕微孢子虫分离株进行草地贪夜蛾Sf9细胞感染性检测,结果显示二者对细胞的侵染能力存在显著差异.为进一步探讨不同家蚕微孢子虫分离株的种群多态性,进行了孢子虫核糖体DNA序列的测定和系统聚类比较,结果表明SSU rDNA(Small subunit ribosomal DNA)和ITS(Internal transcribed spacer)在不同地域的种群差异性并不显著.通过检索重庆株全基因组数据及其他地域株rDNA序列,显示在家蚕微孢子虫rDNA元件的部分SSU rDNA结构复制子中存在MITE-like转座元件的插入,表明家蚕微孢子虫rDNA结构的多样性.%The structure and organization of mitochondrial genomes of 14 marine bivalves and two freshwater bivalves were analyzed using comparative genomics and bioinformatics methods. The results showed that the organization and gene order of the mitochondrial genomes of these bivalve species studied were different from each other. The size, organization, gene numbers, and gene order of mitochondrial genomes in bivalves at different taxa were different. Phylogenetic analysis using the whole mitochondrial genomes and all the coding genes showed different results--phylogenetic analysis conducted using the whole mitochondrial genomes was consistent with the existing classification and phylogenetic analysis conducted using all coding genes not consistent with the existing classification.