植物基因组DNase Ⅰ超敏感位点的研究进展

摘要

Eukaryotes's gene expression and regulation relies on the interaction of their cis-acting elements and trans-acting factors.The cis-elements are specific DNA sequences in the genome and frequently located in the untranslated regions.The trans-acting factors are usually considered to be proteins that bind to the cis-acting sequences to regulate gene expression.It is well known that the cis-elements are always associated with DNase Ⅰ hypersensitive site,which is a signature of open chromatin.The identification of all the functional cis-element using high-throughput method in plant genome has not been initiated in plant genomes.With the rapid achievement of genomics studies,an increasing number of plant genomes have been sequenced.Genome-wide identification of DNase Ⅰ hypersensitive sites will be a considerably efficient method to locate cis-element in plants,which will provide a vital potential for further plant functional genomics.The present review is to reveal the recent progresses on identification and analysis of DNase Ⅰ hypersensitive site in plant genomes.%真核生物的基因表达与调控是一系列顺式作用元件(cis-acting element)与反式作用因子(trans-acting factor)相互作用的结果.顺式作用元件本身不编码蛋白,只是基因组中一些特定的区域,它们需与反式作用因子结合才能发挥其生理作用.目前已知,顺式作用元件往往与染色质的结构密切相关,能与反式作用因子结合发挥生物学功能的顺式作用元件,通常存在于脱氧核糖核酸酶Ⅰ超敏感位点(DNase Ⅰ hypersensitive site)的内部或周围.随着越来越多的植物基因组测序的进展,大规模高通量鉴定植物基因组的顺式作用元件正在受到关注,通过鉴定脱氧核糖核酸酶Ⅰ超敏感位点可以有效确定顺式作用元件的富集区域,将对功能基因组学研究提供的数据支持.文章综述了近期高通量鉴定和分析植物基因组脱氧核糖核酸酶Ⅰ超敏感位点研究进展.