目的 对新疆一枝蒿药材的水分、总灰分、药材鉴别和酮酸含量测定等方面进行研究,建立新疆一枝蒿药材的质量标准.方法 采用2005年版一部附录Ⅸ H 水分测定法中的烘干法和附录Ⅸ K 灰分测定法中总灰分测定方法检查药材的水分和总灰分;采用附录Ⅵ B薄层色谱法建立定性鉴别一枝蒿药材的方法;采用高效液相色谱法(附录Ⅵ D)测定新疆一枝蒿药材活性成分酮酸的含量.结果 新疆一枝蒿药材的含水量不得超过8.0%,总灰分不得超过16.0%;薄层色谱斑点清晰、专属性强;酮酸在5.60～56.00 μg范围内线性关系良好,回归方程Y=8.522 3X-6.150 7,r= 0.997 2,平均加样回收率为99.7%,RSD=0.25%.结论 该方法操作简便、准确快速、重复性好,有助于新疆一枝蒿药材的质量控制,可为药用植物资源的开发利用提供科学依据.%Objective To establish the quality standards for Artem isia rupestris L. fron Xinjiang province by determining water content, total ash, crude material identification and rupestonic acid quantification Methods Moisture and total ash were determined based on the procedures recorded in the AppendixⅨ H, Ⅸ K of phamacopoeia, published in 2005. And the Artem isia rupestris L. was identified by using a thin layer chromatography( TLC) method ( the AppendixⅥ B). and the bioactive component rupestonic acid was detemined by HPLC ( the Appendix Ⅵ D) , respectively. Results The water contained in Artem isia rupestris L. was less than8.0％ , and the total ash was lower than 16. 0％. The TLC spots were clear and specific; the rupestonic acid showed a good linear relationship at a range of 5.60-56.00 μg with the regression equation asY = 8.522 3X-6. 1507, r= 0.997 2 respectively And the average recovery was99.7％ ( RSD=0. 25％ ). Conclusion The method is easy, accurate, quick and reproducible, which is benefit for the quality control of Artem isia rupestris L. , and providing scientific basis for development of Artem isia rupestris L.
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