Objective To observe the effect of puerarin injection on cultured cardiocytes in the perspective of oxidative stress,and explore the mechanism of myocardial protection. Methods Neonatal cardiocytes were cultured and the cylotoxicity in cultured cardiocytes was induced by anoxia. Then, a method of serum pharmacology was used to study the effect of puerarin injection on cell viability, which was analyzed using the MTT assay. Super oxide dismutase (SOD) , malonaldehyde (MDA) , lactate dehydrogenase ( LDH) ,creatine kinase (CK) and creatine kinase MB ( CK-MB) activity in medium were assayed by automatic biochemistry analyzer. Results The absorbance (1) values,LDH,CK,CK-MB,SOD activity and MDA levels of the model group and puerarin injection group were as followes: A values (0. 503 ±0. 014) , (0. 539 ±0. 012) ; LDH (61. 58 ±3.41), (53.54+2.01) U·L-1;CK ( 12. 61 ± 1. 02) , (7. 49±0. 44) U·L-1; CK-MB (6. 30 ± 0. 42) , (2. 52±0. 44) U·L-1;SOD (24. 31 ±0.63), (25. 34±0. 34) U·L-1; MDA (1. 39±0. 22) , ( 1. 08±0. 11 ) μmol·L-1, respectively (P<0.05 or P< 0. 01). The A values indicated that the puerarin injection increased the activity of the cells significantly as compared with the model group. And it could significantly reduce LDH, CK, CK-MB activity and MDA levels and increase SOD activity in the medium as compared with model group ( P<0. 05 or P<0. 01). Conclusion Puerarin injection can inhibit oxidative stress in myocardial cells, thereby to protect myocardium.%目的 从氧化应激角度探讨葛根素注射液对心肌细胞的保护作用机制.方法 建立大鼠乳鼠体外心肌细胞损伤模型.设空白对照组,模型组,葛根素注射液低、中、高剂量组(终浓度分别为为25,50,100 mg·L-1),观察葛根素注射液对细胞活力的影响.利用全自动生化分析仪测定细胞上清液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量.结果 模型组和葛根素注射液高剂量组心肌细胞悬液吸光度(A值)分别为(0.503±0.014),(0.539±0.012)；LDH分别为(61.58±3.41),(53.54±2.01)U·L-1；CK分别为(12.61±1.02),(7.49±0.44)U·L-1；CK-MB分别为(6.30±0.42),(2.52±0.44) U·L-1;SOD分别为(24.31±0.63),(25.34±0.34)U·mL-1；MDA分别为(1.39±0.22),(1.08±0.11)μmol ·L-1.葛根素注射液可明显提高缺氧损伤心肌细胞的活力,能降低LDH、CK、CK-MB活性,降低MDA浓度,提高SOD活性(P＜0.05或P＜0.01).结论 抑制氧化应激是葛根素注射液保护心肌细胞的作用机制之一.
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