目的了解商陆皂苷甲对小鼠急性毒性特点,观察其对大鼠的利尿作用。方法向小鼠腹腔注射不同浓度的商陆皂苷甲,观察给药后小鼠毒性反应,测定急性毒性的相关参数。向水负荷状态大鼠腹腔注射不同剂量商陆皂苷甲,测定给药后连续6 h总尿量。结果 Bliss法计算商陆皂苷甲LD50为26.19 mg·kg-1,95%可信区间为23.11~29.85 mg·kg-1。随着商陆皂苷甲浓度的增加,小鼠的毒性反应表现越明显,且小鼠的死亡数随之增加,空白对照组小鼠无异常反应。高剂量组大鼠尿量与阴性对照组差异有统计学意义,而中、低剂量组差异无统计学意义。结论商陆皂苷甲单次腹腔注射对小鼠有一定的毒性且商陆皂苷甲高剂量对大鼠有利尿作用。%Objective To understand the intensity and characteristics of acute toxicity of esculentoside A on mice and measure relevant parameters and observe its diuresis effect on rat. Methods After intraperitoneal injection of different concentrations of esculentoside A to mice, toxic reactions were observed. Rats with water load were intraperitoneally injected with different doses of esculentoside A. Total urine volume in six consecutive hours after the injection was determined. Results The LD50 of esculentoside A calculated by Bliss method was 26. 19 mg · kg-1 , and the 95% confidence interval was 23. 11-29. 85 mg·kg-1 . The mortality and acute toxicity of esculentoside A appeared to be dose-dependent while the blank control group had no abnormal reaction. The urine volume was significantly different between high dose group and the negative control group. No significant difference in urine volume was found between middle and the negative control group, and between low dose group and the negative control group. Conclusion Esculentoside A is poisonous to mice when single dose was intraperitoneally injected, and high dose of esculentoside A has diuresis effect on rat.
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