瑞香狼毒叶提取物对皮肤石膏样毛癣菌和金黄色葡萄球菌的抑制作用

摘要

Objective To study the antibiotic activities of leaf extract of Stellera chamaejasme L on the skin. Methods Activities of the leaf extract on Trichophyton gypseum and Staphylococcus aureus was examined by antibiotic susceptibility paper disk method and tube double dilution method. Results When extracted by 95%ethanol,the extract from Stellera chamaejasme L at 100 mg·mL-1 formed an inhibition zone with the diameter of 12. 8 mm for Staphylococcus aureus and 12. 0 mm for Trichophyton gypseum. When extracted by dichloromethane,the minimum inhibitory concentration( MIC)of the extract from Stellera chamaejasme L was 0. 25 mg·mL-1 for Staphylococcus aureus and 0. 50 mg·mL-1 for Trichophyton gypsem. Conclusion Leaf extract of Stellera chamaejasme L has an antibacterial activity against Staphylococcus aureus and Trichophyton gypseum and the antibacterial activity against Staphylococcus aureus is stronger.%目的：研究瑞香狼毒叶提取物对常见皮肤表面菌的抑菌活性。方法采用药敏纸片法和二倍稀释法测定瑞香狼毒叶提取物对石膏样毛癣菌和金黄色葡萄球菌的抑菌作用。结果瑞香狼毒叶95％乙醇提取物浓度为100 mg·mL-1时对金黄色葡萄球菌抑菌圈直径达12.8 mm，对石膏样毛癣菌抑菌圈直径达12.0 mm，二氯甲烷萃取物对金黄色葡萄球菌最低抑制浓度（ MIC）为0.25 mg·mL-1，对石膏样毛癣菌MIC为0.50 mg·mL-1。结论瑞香狼毒叶提取物对石膏样毛癣菌和金黄色葡萄球菌均有抑制活性，其中对金黄色葡萄球菌抑制活性较强。