气相色谱-质谱法测定香桂化浊胶囊中桂皮醛的含量

摘要

目的：建立气相色谱-质谱法测定香桂化浊胶囊中桂皮醛含量。方法采用 HP-5弹性石英毛细管色谱柱(30 m×0.25 mm,0.25μm),载气：氦气,流速：1.0 mL·min-1,分流比：50∶1,程序升温,进样量1.0 μL。结果香桂化浊胶囊中桂皮醛和其他成分的分离度良好,在0.02~4.00 mg·mL-1范围内线性关系良好,相关系数为0.9994。桂皮醛的平均加样回收率为96.2%,RSD<2.11% 。结论该方法操作简便,定量准确,适用于香桂化浊胶囊中桂皮醛的含量测定。%Objective To establish a method for determining cinnamic aldehyde content in Xianggui Huazhuo capsules by gas chromatography-mass spectrometry (GC-MS). Methods The content of cinnamic aldehyde was determined by GC-MS. Separation was performed on a capillary column (30 m×0. 25 mm, 0. 25 μm) with HP-5 as the stationary phase. A programmed temperature was employed. The flow rate was 1 mL·min-1 with He as carrier gas, and split ratio was 50∶1. The injection volume was 1. 0 μL. Results The cinnamic aldehyde was well isolated from the other ingredients. A good linear relationships of cin-namic aldehyde in range of 0. 02-4. 00 mg·mL-1 was observed. The correlation coefficient was 0. 999 4. The average recovery of cinnamic aldehyde was 96. 2% , and RSD was less than 2. 11% . Conclusion The method is simple, accurate and suitable for determination of cinnamic aldehyde content.