急性子提取物抗炎镇痛作用的实验研究

摘要

Objective To investigate the anti-inflammatory and analgesic effects of extracts from Garden Balsam seeds in order to provide theoretical basis for rational clinical application. Methods The mouse ear swelling induced by xylene, the body torsion induced by acetic acid or hot plate method, the rat foot swelling induced by egg white and granuloma model were set up to study anti-inflammatory and analgesic effects of extracts from Garden Balsam seeds. Results The water extracts at the middle and high doses reduced the body torsion counts of mice (P<0. 05), alleviated the rat toe swelling, but did not affect mice ear swelling. The extracts at the high dose prolonged pain threshold at 60 min (P<0. 01) and 90 min (P<0. 05); the alcohol ex-tracts at the middle and high doses significantly lowered body twisting counts (P<0. 01), attenuated the ear swelling of mice (P<0. 05), remarkably prolonged the pain threshold (P<0. 01)at 30 min, weakened the rat foot swelling, and elevated 60 min pain threshold at the high doses (P<0. 01), but the extracts exerted no effects on the rats granuloma. Conclusion The extracts from Garden Balsam seeds have anti-inflammatory and analgesic effects.%目的：探讨中药急性子的抗炎镇痛效果，为临床合理应用提供理论依据。方法采用二甲苯致小鼠耳肿胀、冰醋酸致小鼠扭体反应、小鼠热板法、蛋清致大鼠足肿胀和肉芽肿实验观察急性子提取物的抗炎镇痛效果。结果与模型对照组比较，急性子水提物中、大剂量组小鼠扭体次数明显减少(P<0.05)，急性子水提物中、大剂量组大鼠足肿胀减轻。中、大剂量急性子水提物对小鼠耳肿胀无效，其中大剂量可延长造模后60 min(P<0.01)和90 min(P<0.05)小鼠疼痛阈值；急性子醇提取物大剂量可显著减少小鼠扭体次数(P<0.01)，减轻小鼠耳肿胀程度(P<0.05)，明显延长30 min疼痛阈值(P<0.01)，减轻大鼠肿胀程度；急性子醇提物大剂量可延长60 min 疼痛阈值(P<0.01)。急性子提取物对大鼠肉芽肿无抑制作用。结论急性子提取物有一定的镇痛效果和抑制急性炎症作用。