小儿热速清颗粒质量标准研究

摘要

目的 建立小儿热速清颗粒的质量标准.方法 采用薄层色谱法(TLC)对小儿热速清颗粒中的大黄、柴胡、连翘、葛根进行定性鉴别;采用高效液相色谱(HPLC)法测定小儿热速清颗粒中黄芩苷的含量.结果 建立了专属性良好的大黄、柴胡、连翘、葛根的薄层鉴别方法.黄芩苷在0.116 2～1.743 0μg范围内呈良好的线性关系,r=1.000 0;平均回收率100.61％,RSD为0.79％(n=6).结论 所建立的定性定量方法简便、准确、重复性好,可用于该制剂的质量控制.%Objective To establish the quality standard of Xiao'er resuqing gantules.Methods Rhei Radix et Rhizoma,Bupleuri Radix,Forsythiae Fructus,Puerariae Lobatae Radix in the granules were qualitatively identified by the method of thin layer chromatography (TLC).The content of baicalin was analyzed by the method of high performance liquid chromatography (HPLC).Results There were good specificities of the TLC method to identify Rhei Radix et Rhizoma,Bupleuri Radix,Forsythiae Fructus,Puerariae Lobatae Radix.The linear range of baicalin was 0.116 2-1.743 0 μg (r =1.000 0).The average of recovery and RSD were 100.61％,0.79％ (n =6),respectively.Conclusion The method established in this study is simple,accurate,reliable and suitable to be applied to quality control for the preparation of Xiao' er resuqing granules.