高效液相色谱法测定调经祛斑片中芍药苷的含量

摘要

Objective:A HPLC method was established for the determination of paeoniflorin in Tiaojingquban Tablets.Methods:In the analysis,a Diamonsil-C18(150mm × 4.6mm,5μm) column,a mobile phase(25%methanol solution) and a UV wavelength of 230nm. Results:A linear range of 0.0284mg/ml~0.1988mg/ml,an average recovery of 99.57% with RSD of 0.77%(n=6).Conclusion:The method of HPLC is simple,rapid and reliable.%目的：建立HPLC法测定调经祛斑片中芍药苷的含量。方法：Diamonsil-C18色谱柱(150mm×4.6mm,5μm)；流动相为甲醇-水（25：75）；检测波长为230nm。结果：线性范围为0.0284mg/ml～0.1988mg/ml，平均回收率为99.57%，RSD为0.77%（n=6）。结论：该方法简便，快速，准确可靠。