辣椒子叶不定芽的诱导与伸长研究

摘要

为了进一步研究辣椒子叶再生体系的影响因素，以辣椒王种子为试材，诱导无菌苗，取子叶作为外植体，探讨了不同浓度6-BA对辣椒子叶诱导不定芽的影响，并进行了不同浓度GA3与6-BA组合对不定芽伸长效果的研究。结果表明：辣椒子叶在MB5＋0．8 mg·L-1 IAA＋8．0 mg·L-16-BA＋4．0 mg·L-1 AgNO3培养基上诱导不定芽效果最好；MB5＋0．8 mg·L-1 IAA＋5．0 mg·L-16-BA＋2．0 mg·L-1 GA3＋4．0 mg·L-1 AgNO3培养基有利于不定芽的伸长。%In order to further study the influence factors of cotyledon regeneration system of in vitro Capsicum annuum L .,‘Lajiaowang’ seeds were used to induce in vitro plantlets(Capsicum annuum L .) .The differences of adventitious buds induction were compared among the different concentrations of 6-BA .In the meanwhile , the effects of the different concentrations of GA3 and 6-BA combinations on elongation were analyzed .The re-sults showed that the best medium for adventitious buds induction by hypocotyls was MB 5 +0 .8 mg·L-1 IAA+8 .0 mg·L-1 6-BA+4 .0 mg·L-1 AgNO3 .The suitable medium for the adventitious buds elongation was MB5 +0 .8 mg·L-1 IAA+5 .0 mg·L-1 6-BA+2 .0 mg·L-1 GA3 +4 .0 mg·L-1 AgNO3 .