绿豆耐盐愈伤组织的诱导

摘要

In order to research the salt resistance of mung bean varieties ,through the tissue culture technology , the mung bean seed was cultured in the MS medium to get the sterile seedlings ,and then serve the cotyledons , hypocotyl ,embryo root as the explant into dedifferent inductive medium (according to add nutrients divided in‐to eight kinds)and produced embryonic callus ,and making the callus suffered from different salt stress process‐ing that medium added the NaCl with different concentration .The results showed that when NaCl concentration increased from 0 .2% to 1 .0% ,callus volume decreases and color burn became to yellowish brown and black to death .Contained 1 .0% NaCl induction medium ,the group of gradually turned into brown ,eventually die;the survival callus ,cotyledon ,hypocotyl ,and radicle in the 0 .6% NaCl induction were transferred to contain 0 .6% ,0 .8% and 1 .0% NaCl choose medium (MS+6‐BA 0 .2 mg·L‐1 + NAA 0 .6 mg·L‐1 ) ,salt resistance of callus was compared and identified .The results showed that the best salt stress concentration for radicle ,hypo‐cotyl and cotyledon callus were 0 .4% ,0 .8% and 0 .6% respectively .Therefore ,salt resistance of hypocotyl was better than cotyledon ,radicle was the worst .%为探讨绿豆品种的耐盐性，利用离体培养技术，将吉绿3号绿豆种子在MS培养基中培养获无菌苗，然后将其子叶，下胚轴，胚根分别做为外植体放入诱导培养基产生胚性愈伤组织，培养基中放入的不同浓度NaCl。对愈伤组织盐胁迫处理，结果表明：当NaCl浓度由0．2％增加到1．0％时，愈伤组织的体积减小，颜色加深，由绿色变至黑褐色至死亡。在含有1．0％ NaCl诱导培养基上的愈伤组织逐渐变成褐色，最终枯死；将经过0．6％ NaCl诱导培养存活的愈伤组织、子叶、下胚轴、胚根分别转入含有0．6％、0．8％、1．0％ NaCl的选择培养基（MS＋6‐BA 0．2 mg·L‐1＋NAA 0．6 mg·L‐1）上，进行比较，鉴定愈伤组织的耐盐性。结果显示：胚根、下胚轴、子叶愈伤组织的最佳盐胁迫浓度分别为0．4％、0．8％、0．6％。因此，下胚轴的耐盐性最好，子叶一般，胚根最差。