为筛选出糯性小麦材料，研究糯性小麦籽粒的出现频率，明确各分离群体的Wx基因型，使筛选的糯性与非糯性材料呈现出表现型与基因型的统一，以生产上大面积推广应用的普通小麦品种克旱16、克春5号为母本，糯性小麦品种宁糯1号、南农09P18为父本4个杂交组合的 F2代分离群体为研究对象，以半籽粒碘染色与分子检测法为研究手段，对F2分离群体进行糯与非糯性材料鉴定与筛选。结果表明：组合克旱16／宁糯1号、克春5号／宁糯1号、克旱16／南农09P18、克春5号／南农09P18糯性小麦的出现频率分别为1．1％、1．4％、1．4％、1．3％；从4个组合的F2群体100个单株中筛选到了共11株Waxy基因全缺型的植株，与碘染色结果一致。%In order to screen waxy wheat ,study frequency of waxy wheat grain ,determine Wx genotype of seg‐regation population ,presented unification of phenotype and genotype ,taking wheat varieties ( Kehan 16 and Kechun 5) as female parents ,waxy wheat varieties (Ningno 1 ,Nannong 09P18) as male parents ,identification and screen of waxy and non‐waxy wheat from F2 separation population of 4 cross combinations was studied with half grain idodine dyeing and molecular examination .The results indicated that the waxy wheat appear‐ance frequency of Kehan 16/Ningnuo 1 ,Kechun 5/Ningnuo 1 ,Kehan 16/Nannong 09P18 and Kechun 5/Nan‐nong 09P18 were 1 .1% ,1 .4% ,1 .4% ,and 1 .3% respectively .there was 11 plants with waxy genes all deletion were screened from 100 individual plants of F2 generation population of four cross combinations by using the Wx molecular mark identification ,this result obtained the confirmation by the idodine dyed way .
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