CD133β-catenin及hTERT在胃癌组织中的表达及其临床关联性

摘要

Objective:To study the expression of CD133, β-catenin and hTERT in gastric carcinoma and the clinical relevance. Methods:CD133 β-catenin and hTERT expression in tissue samples of 60 cases of gastric cancer ( group A) , 60 cases of precancerous lesions ( group B) and 40 cases of chronic non-atroph-ic gastritis ( group C) admitted in our hospital from February 2015 to March 2016 were detected by immuno-histochemical method and analyzed. Results:The results of immunohistochemistry showed that the positive rates of CD133, β-catenin and hTERT in gastric cancer and precancerous lesions were significantly higher than those in gastritis, the difference was statistically significant ( P < 0.05) , while the positive rate of gastric cancer was significantly higher than that of cancer precancerous lesion. The expression of A, B group CD133,β-catenin beta and hTERT protein was significantly higher than that in group C, while the A group was signif-icantly higher than that in B group, the differences were statistically significant ( P <0.05);CD133 protein in cancer tissues,β-catenin protein and hTERT protein in higher than that without lymph node metastasis in lymph node metastasis and expression. In the depth of invasion in T3+T4 was higher than that in T1+T2. The difference was statistically significant ( P <0.05) . The correlation analysis showed that the expression levels of CD133, β-catenin and hTERT were positively correlated. Conclusion:The expression of CD133,β-catenin and hTERT are closely related to the stomach cancer occurrence, development, invasion and metastasis, which can be used as a clinical reference indexes of early diagnosis and prognosis of gastric cancer.%目的:研究CD133、β-catenin及hTERT在胃癌表达及其临床关联性.方法:采用免疫组织化学法分别检测从2015年2月至2016年3月,我院病理科收集的60例胃癌(A组)、60例癌前病变(B组)以及40例慢性非萎缩性胃炎(C组)组织标本中CD133、β-catenin及hTERT表达情况,并进行相关性分析.结果:免疫组化结果显示:CD133、β-catenin及hTERT在胃癌以及癌前病变中的阳性率均显著高于胃炎,差异有统计学意义(P<0.05),而胃癌中阳性率又显著高于癌前病变.A、B组的CD133、β-catenin及hTERT蛋白表达显著高于C组,而A组又显著高于B组,差异均有统计学意义(均P<0.05);肿瘤组织中CD133蛋白、β-catenin蛋白以及hTERT蛋白在淋巴结转移中表达高于无淋巴结转移,而在浸润深度T3+T4中表达高于T1+T2中.差异均有统计学意义(均P<0.05).经相关性分析可得,CD133、β-catenin及hTERT蛋白表达水平均呈显著正相关.结论:CD133、β-catenin及hTERT的表达@有胃癌发生、发展、浸润、转移均存在密切相关,可作为临床上早期诊断胃癌以及预后的参考指标.