褪黑素对内毒素血症大鼠TNF-α表达的影响

摘要

Objective To investigate the application value of combined detection of alpha-fetoprotein (AFP) .human hepatocellular carcinoma antigen(PHCA) ,and carbohydrate antigen 19-9(CA19-9) in serum in the diagnosis of hepatocellular carcinoma. Methods The three indexes including AFP, PHCA and CA19-9 were detected in 38 cases of HCC( HCC group) ,36 cases of cirrhosis( cirrhosis group) ,and 40 healthy subjects( normal control group) .and the diagnostic value of combined detection for HCC was evaluated. Results The serum levels of AFP,PHCA and CA19-9 in HCC group were significantly higher than those in cirrhosis group( P <0.05). The area under the curve of the three indexes was 0. 872,0. 839,0. 649,respectively,and the critical value of PHCA was 25.79U/L according to ROC curve. In the HCC group, the diagnostic sensitivity of combined detection of AFP, PHCA and CA19-9 was 92. 3% .which was significantly higher than that of single detection of AFP(71. 8% ), PHCA( 71.8% ) or CA19-9 (61. 5% ) ( P < 0. 05). Conclusion The combined detection of AFP, PHCA and CA19-9 can enhance the diagnostic sensitivity and improve diagnostic level for HCC.%目的 探讨褪黑素(MT)对脂多糖(LPS)诱导的内毒素血症大鼠循环血和血管组织中肿瘤坏死因子-α (TNF-α)表达的影响.方法 雄性SD大鼠,分组如下:(1)溶剂对照组；(2)LPS组；(3)LPS+MT组；(4)MT组.应用酶联免疫吸附法检测血清TNF-α含量；应用RT-PCR和免疫组织化学法检测TNF-α在血管组织的表达.结果 与对照组相比,LPS组大鼠血清TNF-α含量、血管TNF-α mRNA水平均增高(P＜0.01),TNF-α蛋白在血管内皮和平滑肌细胞阳性表达增多；而给予LPS前后应用MT干预治疗,可显著降低血清TNF-α含量、血管TNF-α mRNA生成,与同时间点LPS组比较均差异有统计学意义(P＜0.01).结论 MT降低内毒素血症大鼠TNF-α生成和表达.