黄芪多糖对免疫功能影响的体外实验研究

摘要

Objective To investigate the effect of Astragalus Polysaccharides on the immune function of mice ,provide a theoretical basis for its clinical application . Methods The mice spleen cells , which placed in 96-well cell culture plates , were divided into astragalus polysaccharide group and blank control group .The cell proliferation activity was measured by WST-1 method while ELISA method for cell factor IL-2, IFN-γ;flow cytometry (FCM) to detect cell phenotype .Results The proliferation of cell activity of Astragalus polysaccharide group were obvious higher than that of control group ( P <0.01);the concentration of IL-2 and IFN-γin astragalus polysaccharide group increased with the rising concentration of Astragalus polysaccharide , a dose dependent manner in a certain range; astragalus polysaccharides can stimulate the proliferation of B lymphocytes and T lymphocyte .Conclusion Astragalus polysaccharide can enhance immune function .It can not only improve the humoral immune function , but also can increase the cellular immune function .%目的：探讨黄芪多糖对小鼠免疫功能的影响，为黄芪多糖的临床应用提供理论依据。方法取小鼠脾细胞，置于96孔细胞培养板中，设黄芪多糖组和空白对照组， WST-1法测细胞的增殖活性；ELISA测细胞因子IL-2、IFN-γ；流式细胞术（FCM）检测细胞表型。结果黄芪多糖组细胞增殖活性显著高于空白对照组（ P ＜0ⅱ．01）；黄芪多糖组IL-2、IFN-γ的浓度随黄芪多糖浓度的增加而增多，在一定浓度范围内呈剂量依赖关系；黄芪多糖既能刺激B淋巴细胞增殖，也能刺激T淋巴细增殖。结论黄芪多糖可增强免疫功能，它不仅能提高体液免疫功能，而且能提高细胞免疫功能。