免疫多糖对日本蟳抗溶藻弧菌能力的影响

摘要

通过对日本蟳(Charybdis japonica)分别注射生理盐水(对照组)、溶藻弧菌(攻毒组)、免疫多糖(免疫组)、免疫多糖+溶藻弧菌(免疫攻毒组)后，再测定4组肝胰腺及肌肉中溶菌酶(LZM )和超氧化物岐化酶(SOD)的活性变化，探讨免疫多糖对日本蟳抗溶藻弧菌能力的影响.结果表明：日本蟳肝胰腺中 LZM 和SOD 的酶活均高于肌肉中的酶活，且免疫多糖对肝胰腺的免疫增强效应显著高于肌肉；免疫组肝胰腺和肌肉中 LZM 酶活，在注射后24 h 时显著升高至峰值(P<0.05)，分别为(11.2±2.78)U /mg 和(3.75 ± 1.05)U /mg ，肝胰腺和肌肉中的 SOD 酶活也在24 h 达到最高值，分别是对照组的2.2倍和 1.4倍；免疫攻毒组在72 h时，肝胰腺和肌肉中的 LZM 酶活分别高出攻毒组62.8%和75.2%，两组织中 SOD 酶活同样显著高于攻毒组(P<0.05)，是攻毒组的 3.5倍和4.2倍 . 由此可见，免疫多糖能提高日本蟳抗溶藻弧菌能力，且在72 h 达到最佳免疫保护效果.%In order to explore influence of immunopolysaccharide on resistant to V ibrio alginolyticus ofCharybdis j aponica ,C .j aponicas were injected with saline(control group) ,V .alginolyticus (bacte-rial infected group) ,immunopolysaccharide (immune group) ,immunopolysacchride and V . algino-lyticus (immune plus bacterial infected group) ,and then the changes of the lysozyme (LZM ) and su-peroxide dismutase (SOD) in the hepatopancreas and muscle were assaied .The results showed as fol-lows the activities of LZM and SOD in hepatopancreas were higher than in the muscle ,and the im-mune enhancement effect on the hepatopancreas was significantly higher than on the muscle . The LZM's activities of the immune group in the hepatopancreas and muscle were significantly elevated to the peak(P < 0 .05) after C .j aponica were injected at the 24h ,which were (11 .2 ± 2 .78)U /mg and (3 .75 ± 1 .05) U /mg .And SOD's activities of in the hepatopancreas and muscle also reached the peak at the 24h ,which were 2 .2 and 1 .4 times as high as that of control group .At the 72h ,LZM's activi-ties in the hepatopancreas and muscle of the immune plus bacterial infected group were 62 .8% and 75 . 2% higher than those of the bacterial infected group .The SOD's activities were also significantly high-er than those of bacterial infected group(P < 0 .05) ,which were 3 .5 and 4 .2 times as high as that of the bacterial infected group .Therefore ,immunopolysaccharide makes a effective influence on resistant to V ibrio alginolyticus of C .j aponica ,and it can get the optimal immune protection at 72h .