纤维蛋白原在乳腺癌患者血清及癌组织中的表达及临床意义

摘要

Objective To analyze the expression of fibrinogen (PFC) in serum and tumor tissues of patients with breast cancer and its clinical significance .Methods A total of 62 patients with breast cancer treated by surgery in our hospital from September 2014 to September 2016 were enrolled in the study .Their clinical and pathological data were collected after admission ,and the expression of PFC in serum was detected by coagulation analyzer within 7 days be-fore surgery .The cancer tissues were collected as surgery to detect the expression of PFC by immunohistochemical method .The correlation between serum PFC level ,expression of PFC in cancer tissues and clinical and pathological indexes and the correlation between the expression of PFC in serum and cancer tissues were analyzed .Results Before surgery ,the serum PFC level was (3 .41 ± 0 .63) g/L .The increase of PFC level was related to age ,menopause , tumor TNM staging ,tumor diameter ,axillary lymph node metastasis and the increase of metastases (P<0 .05) .PFC protein was positively expressed in breast cancer tissues and metastatic lymph nodes ,and the score of PFC protein expression in cancer tissues was (6 .24 ± 1 .23) .The high expression of PFC protein in breast cancer was related to age ,menopause ,increase of histological grade ,decreased expression of estrogen receptor (ER) and progesterone re-ceptor (PR) (P<0 .05) .Spearman correlation analysis showed that there was no significant correlation between the expression of PFC in serum and cancer tissues beforesurgery (r=0 .768 ,P=0 .325) .Conclusion The levels of serum PFC in patients with breast cancer are closely related to tumour progression .The expression of PFC in breast cancer tissues is not related to the level of serum PFC .Breast cancer tissues may participate in tumor progression and lymph node metastasis by autologous secretion of PFC .PFC is an important molecular marker of proliferation of breast cancer .%目的 分析纤维蛋白原(PFC)在乳腺癌患者血清及癌组织中的表达及临床意义.方法 纳入62例乳腺癌行手术治疗患者临床病理学资料,术前7 d内采用凝血仪检测血清PFC表达水平,术后获得的癌组织标本采用免疫组化法检测PFC蛋白表达情况,分析血清PFC水平、癌组织PFC蛋白表达与临床病理学指标的关系,以及血清PFC与癌组织PFC蛋白表达的相关性.结果 术前血清PFC水平(3.41±0.63)g/L,PFC水平上升与年龄增长、绝经、肿瘤TNM分期进展、肿瘤直径、腋窝淋巴结转移、转移数目增加均相关(P<0.05);乳腺癌癌组织、转移淋巴结组织切片PFC蛋白均为阳性表达,癌组织PFC蛋白表达赋分(6.24±1.23)分,乳腺癌PFC蛋白高表达与年龄增长、绝经、组织学分级升高、雌激素受体(ER)、孕激素受体(PR)表达减少相关(P<0.05);Spearman相关性分析显示,术前血清PFC和癌组织PFC蛋白表达无显著相关性(r=0.768,P=0.325).结论 乳腺癌患者血清PFC水平与肿瘤进展密切相关,PFC在乳腺癌组织中表达与血清PFC水平无关,乳腺癌组织可能通过自体分泌PFC方式参与肿瘤发展和淋巴结转移,PFC是乳腺癌增殖的重要分子标记物.