为了在基因水平上给动物的营养代谢研究提供基础性资料,为系统研究剑白香猪这一优质猪种提供参考,以剑白香猪肌肉组织中的总RNA为模板,克隆了剑白香猪的GLUT-4cDNA序列,并对其序列进行了分析.结果表明:克隆片段总长为1616bp,包含一个长1530bp的开放阅读框(ORF),编码509个氨基酸残基的蛋白；该蛋白的相对分子质量为54.8094kDa,等电点为6.98,包含30个功能位点,即3个N-糖基化位点、1个cAMP和cGMP依赖性蛋白激酶磷酸化位点、4个酪蛋白激酶Ⅱ磷酸化位点、2个酪氨酸激酶磷酸化位点、4个蛋白激酶C磷酸化位点、2个酰胺化位点、12个N-豆蔻酰化位点、1个糖转运蛋白signature 1位点和1个糖转运蛋白signature 2位点；剑白香猪与牛、兔、小家鼠、人、马、黑猩猩、褐家鼠、非洲爪蟾GLUT-4基因编码序列的同源性分别为91.76％、90.52％、87.91％、90.52％、92.29％、90.46％、87.52％和65.82％,氨基酸序列的同源性分别为94.7％、95.68％、94.11％、95.09％、94.89％、95.28％、94.5％和68.31％.%With the purpose of providing the theoretical basis to nutrimental metabolism at the level of gene and offering reference to systematically study Jiangbai Xiang-pig,cDNA of GLUT-4 was amplified by RT-PCR from total RNA of Jiangbai Xiang-pig musculature cell, then the RT-PCR products were cloned and sequenced. The results showed that the length of the fragment was 1 616 bp, containing an ORF of 1 530 bp, which encoded a protein of 509 amino acid residues. The pi of the protein was 6. 98 and its molecular weight was 54. 8094 kDa. Protein prediction shows that the protein contain 30 function sites: three N-glycosylation sites, one cAMPand cGMP-dependent protein kinase phosphorylation site, four casein kinase II phosphorylation sites, two tyrosine kinase phosphorylation sites, four protein kinase C phosphorylation sites, two Amidation sites, 12 N- myristoylation sites, one sugar transporter proteins signaturel, noe sugar transporter proteins signature 2. The coding sequence and the amino acid sequence of GLUT-4 gene from Jiangbai Xiang-pig were compared with those of cattle, rabbit, mouse, human, horse, chimpanzee, norway rat, African clawed frog respectively. The homologies of the coding sequence were 91. 76 %, 90. 52 %, 87.91%, 90.52%, 92.29 %, 90.46%, 87.52% and 65. 82% respectively. The homologies of the amino acid sequence were 94. 7%, 95. 68%, 94. 11%, 95. 09%, 94. 89%, 95. 28%, 94. 5% and 68. 31 % respectively.
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