A protocol is described for rapid in vitro propagation of the valuable chylocaula Opuntia dillenii(Ker-Gawl.)Haw. in vitro. The influence of various combinations of growth regulators,the physical stage of explants and the concentration of the macroelements were also evaluated. It was found that bud variation was dependent on BA supply,the synergistic combination of 5.0 mg/L and 0.1 mg/L IBA induced the optimum frequency(5.3 buds/stem segment). Stem segment with longitudinal split was prior to whole segment on bud induction. The concentration of Ca2+,Mg2+ affected root number and length,but had no effects on plantlet height and growth. Though root length of plantlets declined with increased NAA concentration,0.2 mg/L NAA promoted root number. Considering together,the best rooting medium was half-strength MS. It was observed that sprout tuber was superior to single nodal plate on bud number as well as bud growth. Some morphological variations exhibited in the transferred test-tube plants. This micropropagation procedure may provide the basis for improvement of this chylocaula and was beneficial to the tissue culture of other plants of Cactaceae.%成功建立了仙人掌离体快繁的实验体系,并且对影响微繁殖的一些因素,诸如激素组合、外植体的物理状态、大量元素的含量等进行了研究.结果表明:BA对仙人掌芽增殖具明显作用,MS+BA 5.0 mg/L+IBA 0.1 mg/L为最适增殖培养基;接种方式实验表明劈接优于整棵.钙、镁离子浓度对试管苗生长没有影响,但影响生根数及根长;NAA抑制根的伸长,但一定浓度可促进生根.总体而言,最适的生根培养基为1/2 MS.同时发现块接比单芽接具有优势.试管苗在形态上出现一些变异.实验结果对仙人掌科其它植物的快速繁殖具有参考意义.
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