巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究

摘要

The technological system of cell suspension culture by the flavonoids content of E. Wushanense was established through studing the effect of inoculation concentration, hormone combination, sugar concentration and various medias. The result showed that the suspension culture optimum medium of E. Wushanense was B5 medium supplemented with 1.0 mg· L-1 2,4-D and 0. 2 mg·L-1 6-BA,the sugar concentration was 4. 0% and the optimum inoculation concentration was 2 g per 30 mL. The highest content of the total flavonoids appeared on the medium.%通过研究接种量、激素配比、糖浓度、培养基种类对巫山淫羊藿悬浮培养细胞生长及其愈伤组织黄酮类含量的影响,建立了巫山淫羊藿细胞悬浮培养的技术体系.结果表明:巫山淫羊藿愈伤组织细胞悬浮培养在B5基本培养基中并附加1.0 mg·L-12,4-D和0.2 mg· L-1BA,蔗糖浓度40 g·L-1,接种量每30 mL为鲜重2 g时,黄酮类化合物含量最高.说明用松脆的巫山淫羊藿胚性愈伤组织可容易建立理想的悬浮细胞培养体系.