姜花属种间杂交胚拯救研究

摘要

为提高姜花属种间杂交胚挽救中幼胚萌发率,以白姜花×金姜花的胚珠为试材,研究不同胚珠发育时期、不同培养基及低温处理果实对幼胚萌发率的影响.结果表明,白姜花×金姜花胚挽救的适宜培养基是MS+0.1 mg/L BA十0.1 mg/L NAA；接种时期以60 d的幼胚培养效果最佳；低温处理果实3～6 d能有效提高幼胚的萌发率.%The object of this study was to improve the germination capacity of embryoes of Hedychium hybrids through e mbryo rescue in vitro. Hybrid ovules of H. Coronarium× H. Gardnerianum were used to investigate the effect of different growth stage of embryo,different culture medium and low-temperature treatment on the germination capacity of embryoes. The results indicated that MS medium supplemented with 0. 1 mg/L BA and 0. 1 mg/L NAA showed suitable for embryo rescue in vitro culture. It was showed that the optimum germination capacity of embryoes reached 40. 6%, which was obtained by taking 60 day old immature embryo as explant. Low-temperature treatment of fruits for 3-6 d,had a positive effect on improvement of the germination capacity of embryoes.