高效分子排阻色谱法测定头孢米诺钠聚合物的含量

摘要

Objective?To establish a high molecular exclusion chromatography (HPSEC) method for the determination of sodium cefminox polymer. Methods The efficient gel column;mobile phase (pH 7.0) 0.005mol/L phosphate buffer solution [0.005mol/L disodium hydrogen phosphate:0.005mol/L sodium dihydrogen phosphate (61∶39)];flow rate of 0.5mL/min;detection wavelength 254 nm;injection volume was 10μl;self-control external standard. Results The linear range of the sample measured 0.4-2.5mg/mL (R=0.9995). Conclusion This method can be well separated Cefminox sodium and polymers, simple, rapid, accurate, reproducible, and can be used in the polymer Cefminox sodium test.%目的：建立高效分子排阻色谱（HPSEC）法测定头孢米诺钠聚合物。方法采用高效凝胶色谱柱；流动相为pH 7.0的0.005mol/L的磷酸盐缓冲液[0.005mol/L的磷酸氢二钠：0.005mol/L的磷酸二氢钠（61∶39）]；流速0.5mL/min；检测波长254nm；进样量为10μL；自身对照外标法定量。结果样品测定的线性范围为0.4~2.5mg/mL（R=0.9995）。结论该方法能够较好地分离头孢米诺钠和聚合物，简便快速，定量准确，重现性好，可用于头孢米诺钠中聚合物的检验。