氯化钆对脓毒症大鼠急性肺损伤的肺保护作用

摘要

目的探讨氯化钆（GdCl3）在脓毒症大鼠急性肺损伤中的肺保护作用及可能机制。方法采用盲肠结扎穿孔术（CLP）建立脓毒症模型。将36只成年Wistar大鼠随即均分为假手术组（ Sham组）、脓毒症组（CLP组）和氯化钆治疗组（GdCl3组），GdCl3组术后经尾静脉注射氯化钆溶液。检测血浆TNF-α、丙二醛（MDA）水平，用HE染色法观察肺组织形态，测定肺湿/干重比,统计方法采用SPSS19.0。结果 GdCl3组血浆TNF-α及MDA水平、肺湿/干重比均显著低于CLP组（P＜0.05），光镜下可见GdCl3组肺组织病理学改变明显减轻。结论 GdCl3可明显减轻脓毒症所致的急性肺损伤的炎性反应。%Objective To explore the lung protective role and the possible mechanism by Gadolinium trichloride in sepsis acute lung injury in rats. Methods Model group by sepsis was established by cecal ligation and puncture(CLP).Thirty-six male Wistar rats were randomly divided into sham operation group(Sham group), sepsis group(CLP group) and Gadolinium trichloride group (GdCl3 group), GdCl3 group were injected gadolinium chloride solution via the tail after intravenous . HE staining optical microscope observation of structural changes in the lungs,wet/dry weight ratios and were measured. Statistical Methods were used SPSS19.0. Results GdCl3 group plasma TNF-α and MDA levels , lung wet / dry weight ratios were significantly lower than the CLP group (P<0.05), the lung histopathological changes were significantly reduced of the GdCl3 group through optical microscope. Conclusion GdCl3 can significantly reduce the inflammatory response of sepsis -induced acute lung injury