Objective To establish a determination method of linderane in Weiyangning pill by HPLC.Methods Ultrasonic was used to extract linderane, Diamonsil C18 (4.6 mm×250 mm, 5 μm) column was used, acetonitrile-water (56∶44) as the mobile phase, detection wavelength was 235 nm, the lfow rate was 1.0 mL/min.Results Linderane in the mass range of 0.018-3.6 μg showed a good linear relationship, the average recovery was 99.81%.Conclusion The method is simple, reliable, and accurate and can be used for quality control of the preparation.%目的:建立了胃疡宁丸中乌药醚内酯的高效液相法(HPLC)测定方法。方法采用超声提取乌药醚内酯,使用Diamonsil C18(4.6 mm ×250 mm,5μm)柱,乙腈-水(56∶44)为流动相,检测波长为235 nm,流速为1.0 mL/min。结果乌药醚内酯在0.018~3.6μg质量范围内呈良好的线性关系,r=0.9995,平均回收率为99.81%。结论该测定方法简便、准确、可靠,可用于胃疡宁丸的质量控制。
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