黄芩苷对哮喘大鼠 p38 MAPK 信号通路影响初探

摘要

Objective To explore the mechanism of Baicalin in treatment of bronchia asthma. Methods Animal models of bronchia asthma were made in rats sensitized with egg albumen.After the treatment of Baicalin,immunohistochemistry and western-blot methods were used to test expression quantity of phosphorylated p38 protein of lung tissue in all groups of guinea rats. Results Our data confirmed that the level of phosphorylated p38 protein increased significantly in model group,but it decreased in hexadecadrol group,high dose and low dose Baicalin group (P <0.05). Conclusion The effects of Baicalin in asthma model were associated with inhibition of P38 MAPK signal pathways in a dose-dependent manner.%目的：初步探讨黄芩苷防治支气管哮喘的作用机理。方法用卵蛋白致敏大鼠制备支气管哮喘动物模型，经黄芩苷干预治疗，运用免疫组化法及 Western Blot 法检测各组大鼠肺组织匀浆中 p38 MAPK 磷酸化蛋白表达量。结果两种检测方法均显示，p38 MAPK 磷酸化蛋白水平在模型组中有明显的增加，地塞米松组、黄芩苷高剂量组和低剂量组的 p38 MAPK 磷酸化蛋白水平均低于模型组（P ＜0．05）。结论黄芩苷能有效治疗哮喘的作用与抑制哮喘大鼠 p38 MAPK 信号通路的表达密切相关。