重金属对藻类的作用及研究机理

摘要

Cladophora in different concentrations of Hg 2 +, Cd2+to the growth conditions was studied , and through the fresh weight determination of Cladophora , chlorophyll content , malondialdehyde content , and protein content , the effect and mechanism of heavy metal on algae was investigated.The experimental data showed that Hg 2 + to the toxic effects of Cladophora in the 0.1 mg/L was their fresh weight and chlorophyll begins to decrease.Cd2+of 5.0 mg/L of heavy seasonal chlorophyll began to decline.Malondialdehyde , protein analysis can know , heavy metal content of Cladophora malondialdehyde increased malondialdehyde content of the control group.0 ~0.5 mg/L protein content , protein content of heavy metals in the body of Cladophora , of Hg2 +experimental group increased by 0.5 ~5.0 mg/L, protein content decreased in the 5.0 mg/L.Cd2+experiments in the 0~10 mg/L, and the protein content increased in the 10~50 mg/L with protein content decreased.Through the analysis , the effect of Hg 2+on the toxicity of cladophora was greater than that of Cd 2 +.%研究刚毛藻在不同浓度的Hg2＋、 Cd2＋下的生长状况，通过生物量、叶绿素、丙二醛、蛋白质这些生化指标的变化来了解重金属对藻类的作用及机理。实验数据表明： Hg2＋的毒害作用较大，在0.1 mg/L时其鲜重和叶绿素已经开始降低。而C（ Cd2＋）＝5.0 mg/L时鲜重叶绿素才开始下降。通过对丙二醛、蛋白质分析知道， Hg2＋、 Cd2＋作用后刚毛藻的丙二醛含量上升，重金属影响刚毛藻体内的蛋白质含量， C（ Hg2＋）在0～0.5 mg/L蛋白质含量上升，在0.5～5.0 mg/L蛋白质含量下降。 C（ Cd2＋）在0～10 mg/L时蛋白质含量上升，在10～50 mg/L蛋白质含量下降，通过分析可知Hg2＋对刚毛藻的毒害作用要比Cd2＋大。