广陈皮DNA条形码的初步研究

摘要

Pericarpium Citri Reticulatae was from the dry and mature skin of Citrus reticulata Blanco and cultivar. According to different sources of medicinal materials, it can be divided into‘orange’ and‘wide dried tangerine or orange peel’ . Citri Reticulatae Pericarpium from Guangdong from‘Chazhi’ was recognized as Chinese drugs. A method on DNA barcode of Citri Reticulatae Pericarpium from Guangdong was established. The trnH-psbA sequences of different samples of Citri Reticulatae Pericarpium were analyzed in order to establish DNA barcode of Citri Reticulatae Pericarpium from Guangdong. So the trnH-psbA DNA barcode can be used to preliminary identification of Citri Reticulatae Pericarpium from Guangdong.%陈皮( Citri Reticulatae Pericarpium )为芸香科植物橘Citrus Reticulata Blanco及其栽培变种的干燥成熟果皮。根据药材来源不同又可分为“陈皮”和“广陈皮”。其中“广陈皮”是变种的茶枝柑果皮，为公认的道地药材。本文拟在分子水平建立广陈皮的DNA条形码，并对来源不同的陈皮样本在trnH-psbA序列进行分析，建立广陈皮的DNA序列。结果表明广陈皮样本与橙类果皮样本之间有明显的差异。因此，判断trnH-psbA序列DNA条形码可用于初步鉴别广陈皮。