穿心莲内酯微球中穿心莲内酯含量测定方法的建立

摘要

A method for the content determination of andrographolide in andrographolide microspheres was established.Using UV spectrophotometry , weighing the appropriate amount of andrographolide , dissolved in 95%ethanol solution diluted in 200~500 nm wavelength scanning , respectively , the maximum absorption wavelength was detected as 225 nm.Results showed that the calculated standard curve was y =29.122 x-0.2382 , R2=0.9992 , with a good linear relationship in 5.0~22.5 μg/mL.The method showed that reproducibility , recovery and stability met the requirement of experiment , and there was no interference from accessories .%建立了穿心莲内酯微球中穿心莲内酯的含量测定方法。采用紫外分光光度法，精密称量穿心莲内酯适量，用95％乙醇溶液溶解稀释，在200～500 nm波长扫描，测得最大吸收波长为225 nm。标准曲线为y＝29．122 x－0．2382， R2＝0．9992，表明在5．0～22．5μg／mL线性关系良好。该方法重现性、回收率和稳定性均符合实验要求，辅料对检测无干扰。