水溶性CdTe纳米棒的制备及在细胞成像中的应用

摘要

High-quality CdTe nanorods were rapidly synthesized in aqueous phase using Na2 TeO3 as tellurium source and two stabilizers ( L-Cys and TGA ) . Different technologies including UV-Vis spectra, fluorescence spectra and scanning electron microscopy ( SEM ) were employed to characterize the prepared CdTe nanorods. The CdTe nanorods capped with two stabilizers exhibited a high uniformity in diameter and excellent fluorescence properties. Further study of imaging cell with the as prepared CdTe nanorods indicated that the L-Cys-TGA-CdTe nanorods can enter the cytoplasma of HepG2. 2. 15 cells. However, the nanorods could not enter the nucleus. The resultant CdTe nanorods have superior fluorescence properties and biocompatibility, which can serve as a powerful fluorescent probe for biosensing and cell imaging.%以亚碲酸钠( Na2 TeO3)为碲源，半胱氨酸( L-Cys)和巯基乙酸( TGA)为双修饰剂在水相中快速合成高质量CdTe纳米棒。通过紫外可见分光光谱仪、荧光光谱仪、扫描电子显微等相关方法对产物进行表征。实验结果表明，所制备的CdTe纳米棒具有强的紫外吸收和高荧光特性及均匀的微米级棒状形貌。更重要的是通过细胞成像发现，尺寸较大的CdTe纳米棒主要进入细胞质中，不能进入细胞核。本文的研究显示： L-Cys/TGA双修饰CdTe纳米棒具有良好的荧光特性和生物相容性，在生物传感和细胞成像领域具有重要的应用价值。