芦荟凝胶的制备及其稳定性评价

摘要

目的 探讨芦荟凝胶的制备工艺并对其稳定性进行评价. 方法 以新鲜库拉索芦荟叶作为原药材,经加工辅以亚硫酸钠制成芦荟凝胶,采用醇沉法提取芦荟多糖,采用蒽酮-浓硫酸紫外分光光度法测定芦荟多糖的含量;评价芦荟多糖在不同pH及温度、日内及日间的稳定性以及芦荟凝胶在室温、恒温及强光下储存4周的稳定性. 采用精密度试验和加样回收率试验测量吸光度值,并考察其稳定性. 结果 芦荟凝胶成品质地均匀细腻,为乳白色透明的混悬稠厚液体,稠度适宜,涂展性好,无特殊气味;芦荟多糖浓度(以D-甘露糖为参照) 在0.05~0.60 g/L范围内有良好的线性关系,回归方程为A=6.1147C+0.0206(r=0.9985,n=6),含量为2.56 mg/g,平均回收率为98.44%,相对标准偏差为1.27%;在不同pH及温度、不同放置时间及天数下芦荟多糖含量比较,差异无统计学意义( P<0.05);芦荟凝胶在室温、恒温及强光下储存3周其外观及含量基本保持稳定,第4周后,其在室温下仍保持稳定,而在恒温和强光下其质地和气味保持稳定,但颜色和黏稠度均有所变化,含量有所增加. 结论 芦荟凝胶性质稳定,适宜常温阴凉处贮藏.%Objective To explore the technology of preparation for aloe gel,and to evaluate its stability.Methods The fresh leaves of aloe barbadensis were taken as original herbs for the process and preparation of aloe gel assisted by sodium sulfite.Alcohol precipitating method was used to extract the polysaccharide of aloe.The content of polysaccharide of aloe was determined using the method of ultraviolet spectrophotometry with anthracenone and sulfuric acid.The stability of polysaccharide of aloe of various pH values,temperatures, times and days of storage was evaluated.And the stability of aloe gel storage at the room temperature,constant temperature and high light for four weeks was also assessed.Precision test and sample recovery experiment were adopted to determine the absorbance,and the stability was observed. Results The finished product of aloe gel had uniform and delicate texture,presented mixed,suspending,heavy and thick liquid appearing,and was ivory white and transparent.It achieved suitable viscosity and good ductility without special smell.The concentration of polysaccharide of aloe had a good liner relationship within the range of 0.05 to 0.60 g/L (D-mannose was taken as the reference).The regression equation was A=6.1147C+0.0206(r=0.9985,n=6),and the content of polysaccharide of aloe was 2.56 mg/g.The average recovery rate was 98.44%,and the relative standard deviation was 1.27%.There were no significant differences in the contents of polysaccharide of aloe among various pH values,various temperatures,various storage timings or various storage durations(P<0.05).The appearance and content of aloe gel remained basically stable at the room temperature,constant temperature and high light after three weeks of storage.In the 4th week,aloe gel remained stable at the room temperature,its texture and smell kept stable at the constant temperature or high light,however,its color and viscosity changed,and its content increased.Conclusion Aloe gel owns the stable quality,and it is suitable for storage at the room temperature and in a cool place.