目的：研究妊娠期女性EB病毒（Epstein⁃Barr virus，EBV）和牙龈卟啉单胞菌（Porphyromonas gingivalis， Pg）的协同感染情况和牙周炎严重程度的关系。方法收集36例患慢性牙周炎（妊娠期慢性牙周炎组）的妊娠期女性和36例牙周健康（妊娠期牙周健康组）的妊娠期女性唾液样本，应用巢式PCR技术检测EBV感染率，应用16S rRNA为基础的PCR技术检测Pg感染率，结合口腔临床检查收集的牙周临床指标，对EBV和Pg的协同感染与牙周炎严重程度的相关性加以分析。结果妊娠期慢性牙周炎组和妊娠期牙周健康组EBV、Pg感染率的检出率差异无统计学意义（P＞0.05），2组EBV和Pg协同感染的检出率差异具有统计学意义（χ2=4.800，P=0.028）。EBV和Pg协同感染与探诊出血指数相关（t=3.058，P=0.003），与牙周袋深度和附着丧失无关（P＞0.05）。结论 EBV和Pg协同感染与妊娠期慢性牙周炎的相关性仍需进一步研究。%Objective To study the correlation between coinfection of Epstein⁃Barr virus (EBV) and Porphyromon⁃as gingivalis(Pg) and severity of maternal chronic periodontitis in pregnant women. Methods Saliva samples were collected from 36 pregnant women with chronic periodontitis and 36 pregnant women with healthy periodontal status. EBV was detected using nested polymerase chain reaction (PCR), Pg was detected using 16S rRNA based PCR. Clini⁃cal periodontal indices were recorded through periodontal examination. Correlation between the coinfection and severity of maternal chronic periodontitis were analyzed. Results The results showed no significant differences in prevalence of infection of EBV or Pg between pregnant women with chronic periodontitis and with healthy periodontal status (P>0.05). Contemporaneously, coinfection prevalence of EBV and Pg showed significant differences between two groups (P = 0.028). Coinfection of EBV and Pg showed significant association with bleeding on probing index (BOP) (P =0.003). No significant associations were found between the coinfection and periodontal depth(PD) or clinical attachment loss (CAL) (P>0.05). Conclusion Relationship between coinfection of EBV and Pg and maternal chronic periodonti⁃tis needs further investigation.
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