顶花板凳果愈伤组织和芽诱导培养研究

摘要

为快速繁殖顶花板凳果优良品种,筛选出顶花板凳果愈伤组织和芽诱导的最佳培养条件,以顶花板凳果当年生鲜嫩带芽茎段为试材,研究了不同采样时间、植体消毒方式、激素配比、是否光照处理等培养条件对顶花板凳果愈伤组织和芽的诱导的影响.结果表明:5月是顶花板凳果最佳采样季节,其外植体的愈伤组织和芽诱导率高于其他月份、为44%;以仅用0.1%HgCl2处理5 min的消毒效果最好,污染率及死亡率均较低,分别为11%、22%;NAA 0.5 mg/L + 6-BA 2.0 mg/L为最佳愈伤组织和芽诱导培养的激素浓度及配比,诱导率高达47%;以光照培养的效果最佳,诱导率为60%.%In order to rapidly propagate elite varieties of Pachysandra terminalis and screen out optimal culture conditions for callus and bud induction ,we studied the effects of different culture conditions on induction of callus and bud in P. terminalis including different sampling time,disinfecting methods of explants,hormone concentration andlight treatment,taking annual tender stem with lateral buds from P. terminalis as explants. The results showed that the best sampling season was May which provided acallus induction and bud induction rate of explants of 44% . Better disinfection effect could be achieved by disinfecting with 0.1% HgCl2for 6 min,of which the pollution rate and the death rate was 11% and 22% respectively. The optimal concentration of hormone for inducing medium of callus and buds was 0.5 mg/L NAA+2.0 mg/L 6-BA,with an induction rate of 47%. Light culture is better than dark culture, with an induction rate of 60%.