Callogenesis and Shoot Organogenesis from Flowers of Stevia rebaudiana (Bert.)

摘要

The callogenic and shoot organogenic potential of the medicinaly important plant, 5. rebaudiana were investigated. Callus induction and shoot regeneration were induced from flower explants of greenhouse plants incubated on Murashige and Skoog (MS)-medium supplemented with different plant growth regulators (PGRs). The best callogenic response was observed on explants incubated on MS-medium supplemented with 2.0 mg/16-benzyladenine (BA) along with 2.0 mg/12,4-dichlorophenoxyacetic acid (2,4-D) after 30-days of culture. Subsequent transfer of callogenic explants onto MS-medium supplemented with 2.0 mg/1 BA gave 85.2% shoot organogenesis after 30-days following culture. Maximum number (21.6) of shoots/explant was recorded for 2.0 mg/1 BA. Subsequent sub culturing of shoots after 3 weeks of culture, on medium with similar compositions of PGRs gave 5.1 cm longest shoot. The pal-ynology of 5. rebaudiana has also been investigated.