瑞香狼毒毒性部位的化学成分研究

摘要

目的：研究瑞香狼毒的毒性部位及毒性部位的化学成分。方法通过小鼠急性毒性实验筛选出瑞香狼毒的毒性部位，采用多种色谱技术分离毒性部位提取物，并根据化合物的理化性质和光谱数据分析鉴别单体化合物的结构。结果确定乙酸乙酯极性部位为狼毒毒性部位，在乙酸乙酯部位中分离得到12个化合物，分别鉴定为：Epiafzelechin (1)、Scopoletin (2)、Isolariciresinol (3)、Lirioresinol B (4)、Stelleranol (5)、Daphnoretin (6)、Chamaechromone (7)、Neochamaejasmin A (8)、Chamaejasmine (9)、Isochamaejasmin (10)、7-methoxyneochamaejasmin A (11)及 Matairesinol (12)。结论本文首次报道了瑞香狼毒的毒性部位研究，并分离鉴定毒性部位的化学成分。%Objective To isolate and identify the compounds of the toxic part from the Stellera chamaejasme L. Methods Toxic part of the Stellera chamaejasme L. was screened by acute toxicity,the compounds were separated and purified by chromatographic technique and their structures were established by spectroscopic methods. Results The toxicity part of the Stellera chamaejasme L. was the polar parts of ethyl acetate,and twelve compounds were obtained from ethyl acetate extracts of the Stellera chamaejasme L. ,which were identified as Epiafzelechin (1), Scopoletin (2), Isolariciresinol (3), Lirioresinol B (4), Stelleranol (5), Daphnoretin (6), Chamaechromone (7), Neochamaejasmin A (8), Chamaejasmine (9), Isochamaejasmin (10), 7-methoxyneochamaejasmin A (11) and Matairesinol (12). Conclusion This is the first report of the toxicity of polar fractions of Stellera chamaejasme L. ,separate and identify the chemical composition of the toxicity of polar parts.