葛根有效成份提取新工艺研究

摘要

Objective To determine the optimum conditions for extracting active ingredients from Pueraria lobata.Methods Using 40 ％ ethanol as solvent, the total flavonoids yield was evaluated by orthogonal experiment, and the best process conditions were selected.Result The optimum extracting conditions of total puerarin flavonoids with 40％ ethanol as solvent, were as follows:extraction time is120 min, solvent dosage is 25 times, extraction temperature is 80℃, extraction times is 2 times.Conclusion In optimum conditions for total puerarin flavonoids in a yield of 8.95％. The experiment methods are convenient, which have strong efficiency and safety, suitable for industrial application.%目的 确定葛根有效成份提取的最佳条件.方法 以40％乙醇为溶剂, 通过正交实验, 以葛根总黄酮得率为评价指标, 筛选最佳工艺条件.结果 40％乙醇为溶剂提取葛根总黄酮的最佳组合条件为提取时间120min, 溶剂用量25倍, 提取温度80℃, 提取次数2次.结论 该条件下葛根中总黄酮的得率为8.95％.此实验方法操作简单、快捷, 具有高效性及安全性, 适合工业化生产推广.