HPLC法测定不同厂家人参北芪片中人参皂苷Rg1的含量

摘要

目的 建立用高效液相色谱法同时测定不同厂家人参北芪片中人参皂苷Rg1的含量. 方法 LC-2010A高效液相色谱仪,Soultion色谱工作站. 色谱柱:YMC-Pack ODS-AC18柱(150×4.6mmID, 5μm),流动相:乙腈 – 0.02%磷酸 ( 20.2:79.8 );检测波长 : 203nm;柱温为 40℃;进样量为10μl;流速为1.0ml/min.结果 结果表明人参皂苷Rg1在0.166~1.192μ范围内线性关系良好.人参皂苷Rg1回收率为97.60%( RSD1.78%). 结论 该方法快捷、可靠、灵敏度高,可用于人参北芪片的含量测定.%Objective To establish a high performance liquid chromatography(HPLC)method fordetermination of ginsenosideRg1 in Renshen Beiqi Tablets of different manufacturers.Method: LC-2010A HPLC and Soultion chromatography workstation. Column:YMC-PackODS-AC18column (150× 4.6mmID, 5μm). Mobile phase: Acetonitrile - 0.02% phosphoric acid (20.2: 79.8) the detection wavelength was set at 203nm; The column temperature was 40 ℃; The injection volume was 10μl; flow rate of 1.0ml /min. The results showed that ginsenosides Rg1 at 0.166 ~1.192μ good linear relationship. Ginsenosides Rg1 recovery was 97.60% (RSD1.78%). Conclusion This method fast, reliable, high sensitivity, can be used for determination of the content of Renshen Beiqi Tablets.