环翠楼高丽红参抗疲劳与抗氧化活性研究

摘要

目的 研究环翠楼高丽红参的抗疲劳和抗氧化活性. 方法 利用小鼠负重游泳实验模型对小鼠体内血乳酸(BLA)和血清尿素氮(BUN)含量和清除率进行测定,对小鼠体内肝糖原(LG)和肌糖原(MG)含量和回复率进行测定,检测环翠楼高丽红参的抗疲劳作用;通过对羟基自由基(OH)和超氧阴离子自由基(O2)的清除能力进行测定来检测环翠楼高丽红参的体外抗氧化活性;对运动后小鼠肝脏中总抗氧化的能力(T-AOC)和超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量进行测定,检测其对运动后小鼠体内抗氧化的功能.结果 环翠楼高丽红参具有较强的抗疲劳作用并对运动后小鼠体内抗氧化作用效果明显(p<0.05),有清除自由基能力,体外抗氧化活性显著.结论 环翠楼高丽红参具有显著的抗疲劳与抗氧化活性.%Objective To study the anti-fatigue and anti-oxidant Activity of Huancui Floor Korea Red Ginseng. Methods to detect Anti fatigue effect of Huancui Floor Korea Red Ginseng, the mice model of weight loading swimming experiment of mice blood lactic acid (BLA) and blood urea nitrogen (BUN) content and scavenging assay rate and liver glycogen (LG)and muscle glycogen (MG) content and recovery rate were detected in vivo. to detect antioxidant activity of Huancui Floor Korea Red Ginseng, the hydroxyl radical (·OH) and superoxide anion free radical (O2-·) scavenging capacity were detected in vitro. to detect antioxidation function after mice exercise,the total antioxidant capacity in liver after mouse movement (T-AOC) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected.Results Huancui Floor Korea Red Ginseng have a strong ability of anti fatigue effect and antioxidant effects obviously after mice exercise (p<0.05) , and scavenging free radical activity and antioxidation activity significantly in vitro (p<0.01). Conclusion the Huancui Floor Korea Red Ginseng has significant anti fatigue activity and antioxidant activity.