含钾矿粉对胶质芽孢杆菌分泌胞外多糖的影响

摘要

研究了不同实验条件下,含钾矿粉对胶质芽孢杆菌(Bacillus mucilaginosus)分泌胞外多糖的影响,以进一步揭示细菌与矿物的相互作用机理.实验分别采用无矿(不加含钾矿粉)无钾(不含可溶性钾)、无矿有钾(有可溶性钾)、有矿(100mL培养液加0.3 g含钾矿粉)无钾及有矿有钾4组培养基培养胶质芽孢杆菌,进行产胞外多糖的分析比较.研究发现,在有钾长石粉但不含钾离子的培养条件下,胶质芽孢杆菌产生的多糖和蛋白质含量最高;在既有钾矿粉又有可溶性钾离子存在的培养条件下,菌体产生的蛋白质和胞外多糖含量稍低;在无钾矿粉的条件下,无论有没有钾离子的两种培养基中,蛋白质和多糖含量都很低.研究结果表明,有无钾矿粉的不同培养条件能够引起胶质芽孢杆菌分泌胞外多糖的单糖组成成分产生明显差异,胶质芽孢杆菌胞外分泌物与细菌对含钾矿粉中钾的利用密切相关.%Under different conditions, effects of mineral powder containing potassium on the exo-polysaccharides (EPS) secreted by Bacillus mucilaginosus were studied to further explain the reciprocity between bacteria and potassium-baring minerals. In the research, B. mucilaginosus was cultured in four kinds of media to analyze and compare their EPS production. Results showed that bacteria secreted the highest output of both proteins and EPS in the culture with potassium-boaring minerals, but without potassium ion. While in the medium with potassium ion, the yielded EPS were lower. However, in the medium without potassium beaing mineral powder and with or without potassium ion, the production of both proteins and EPS was proved to bo the lowest. List of monosaccharide components of EPS secreted by B. mucilaginosus varied obviously in relation with different culture conditions. The results demonstrated that extracellular secretion of the bacteria is closely related to the mechanism of potassium releasing.