云南白药对豚鼠缺血心肌的保护作用

摘要

目的 观察垂体后叶素心肌缺血造模后,不同剂量的云南白药腹腔注射对缺血心肌的保护作用.方法 将30只正常豚鼠随机分为5组,即N组(正常组),ADH组(垂体后叶素0.5 U/kg腹腔注射造模)、NO组(造模后经27.75 μg/kg硝酸甘油解救)、LD组(造模后经低浓度云南白药3.6 mg/kg解救)和HD组(造模后经高浓度云南白药36 mg/kg解救),每组6只.通过测定豚鼠心电图变化和心肌缺血面积并观察心肌细胞组织切片形态变化,比较硝酸甘油和高、低剂量云南白药对于心肌缺血的保护作用.结果 ADH组心电图出现T波,心肌细胞变性,排列紊乱.NO组短时间内使抬高的T波下降,心肌细胞无变性,排列正常.LD组和HD组使抬高的T波下降,历时较长,心肌细胞变性不显著,且HD组较优.结论 云南白药对于缺血心肌有解救作用,且存在剂量效应正相关.%Objective To observe the effect of Yunnan Baiyao powder on the antidiuretic hormone (ADH) induced myocardial ischemia. Methods We randomly divide 30 guinea pigs into 5 groups. N group (normal group), ADH group (myocardial ischemia model induced by 0. 5 units of ADH per kg through intraperitoneal injection) ,NO group (ADH group rescued by 27. 75 fig/kg nitroglycerin) ,LD group (ADH group rescued by 3. 6 mg/kg Yunnan Baiyao powder) , and HD group (ADH group rescued by 36 mg/kg Yunnan Baiyao powder). To compare the protective effect of Yunnan Baiyao powder and nitroglycerin,ECG,ischemic area and morphological changes were observed. Results In ADH group,T wave raised,myocardial cells were degenerated and arranged disorderly. In NO group, raised T wave declined to normal in a short time. The morphology of cells remained normal. In LD group and HD group,the raised T wave declined in a longer time,and the morphology of cells was close to normal. The status of HD group was better than the LD group. Conclusions Yunnan Baiyao powder has rescue effect on ischemiac myocardium in a dose-dependant way.