Isolation andapplicationofanibuprofen-degrading bacterium toabiologicalaerated filter forthetreatmentof micro-polluted water

摘要

Ibuprofen(IBU)is widely used in the world as anti-inflammatory drug,which posed health risk to the environment.A bacterium capable of degrading IBU was isolated from activated sludge in a sewage treatment plant.According to its morphological,physiologic,and biochemical characteristics,as well as 16S rRNA sequence analysis,the strain was identified as Serratia marcescens BL1(BL1).Degradation of IBU required the presence of primary substrate.After a five-day cultivation with yeast powder at 30°C and pH 7,the highest degradation(93.47%2.37%)was achieved.The process of BL1 degrading IBU followed first-order reaction kinetics.The BL1 strain was applied to a small biological aerated filter(BAF)device to form a biofilm with activated sludge.IBU removal by the BAF was consistent with the results of static tests.The removal of IBU was 32.01% to 44.04% higher than for a BAF without BL1.The indigenous bacterial community was able to effectively remove CODMn(permanganate index)and ammonia nitrogen in the presence of BL1.