Cold-tolerant clones of Casuarina glauca were established through vegetative reproduction of the plants survived under low temperature ( - 5-6 ℃) stress. The physiological characteristics of two cold-tolerant clones and the control at 4 low-temperature gradient treatments from -2 ℃ to -11 ℃ were tested. The results showed that the relative conductivity, soluble protein content, MDA content and SOD activity of all clones increased with the temperature decrease. The relative conductivity of two cold- tolerant C. Glauca clones were lower than that of the control, while the soluble protein content, MDA content and SOD activity were higher than that of the control. The relative conductivity, the soluble protein content, MDA content and SOD activity can be used as screening index for the cold tolerance identification of C. Glauca. The Ci 36 clone was stronger cold-tolerant than Ci 24 clone.%从经过低温(-5～-6℃)冻害的粗枝木麻黄林分中选出较耐寒的单株,经无性繁殖成无性系,然后再选择出耐寒无性系.采用-2℃至-11℃低温处理后,2个较耐寒粗枝木麻黄无性系和对照的相对电导率、可溶性蛋白含量、MDA含量和SOD活性生理指标均表现为随着温度降低而升高的趋势,2个较耐寒无性系的相对电导率比对照低,而可溶性蛋白含量、MDA含量和SOD活性则比对照高,它们可用作鉴定粗枝木麻黄无性系耐寒性强弱的生理指标.慈36号无性系比慈24号无性系更耐寒.
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