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蓝莓的组织培养及快速繁殖

             

摘要

对蓝莓的组培快繁技术进行了研究.结果表明:茎段在1/3MS + BA1.5 mg/L + NAA0.2 mg/L+ ZT1.5 mg/L 培养基上继代一次即可出苗,繁苗系数8倍以上;生根培养以1/4MS + IBA0.5 mg/L 培养基为最好,生根率达100%,并且每株可达5~10条根;移栽基质以腐苔藓为最好,成活率达80%以上%  Cell culture was made of the blueberry for its rapid propagation .Orthogonal design indicated that 1/3MS + 6-BA 1 .5mg/L + NAA 0 .2mg/L + ZT 1 .5 mg/L was the best culture medium to induce proliferation buds from its tuber .Propagation coefficient was eight times .The best culture medium for multiplication of cluster shoots was 1/4 MS + IBA 0 .5 mg/L .Rooting rate 100% ,every plant root 5-10 ;When micropropagated plantlets with well-developed root systems were transferred to planting bed of moss in a greenhouse ,80% of the plantlets survived .

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