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文冠果种子浸提液对白菜种子萌发的影响

         

摘要

文冠果种子营养丰富、含油率高。花色品种多样且好看,花期长达20多天。但由于文冠果种子较难萌发,实验主要研究文冠果种子各部位是否含有抑制物,各部位抑制物含量比较,及用赤霉素处理后是否能降低抑制,使其能大面积种植。实验取饱满的文冠果种子的种胚、胚乳、种皮和完整种子各2g,研磨后,分别用蒸馏水和80%甲醇溶液在0℃下静止提取定容,制成浓度为0.5、0.1、0.15g/mL的溶液,倒入培养皿中每皿放100粒白菜种子进行发芽试验,每种处理3次重复。用200g/mL的赤霉素浸泡饱满的文冠果种子2天,再重复上述实验过程。结果表明:文冠果种子的蒸馏水提取液对白菜种子萌发抑制较弱,而甲醇提取液对其抑制较强,被赤霉素处理后抑制作用都有所减弱,但不能完全消除。文冠果种子各部分都含有含有抑制物,其中种皮含量较多。%Xanthocera is nutrient-rich seeds. Xanthocera is China's rare and unique species of woody fuels and civil medicinal plants, Xanthoceras diverse varieties and good-looking, relatively long flowering time, more than 20 days, However, due to difficult Xanthoceras seed germination, so this experiment Xantho- ceras main site if it contains the seeds of the inhibitor, and its content in various parts of the inhibitor con- trast, and after treatment with gibberellic acid can reduce the inhibition. To enable them to large-scale cultivation. Experiment Xanthoceras full seed embryo, endosperm, seed coat and integrity of the seed, after grinding, respectively, with distilled water and 80~ methanol solution at 0 ~C under the static ex- traction volume. To produce a concentration of 0. 5,0.1,0.15g/mL solution, poured into the Petri dish put in 100 per dish cabbage seed germination tests, each repeated 3 times to deal with. 200g/mL with the gibberellin immersion Xanthoceras full two days of seed, to repeat the experimental process of appeal. The results showed that: Xanthoceras the distilled water extract of the seeds of Chinese cabbage seed germination inhibition is weak, while the methanol extract of its strong inhibition by the inhibitory effect of gibberellin treatment have been weakened, but not completely eliminated. Xanthoceras con tain the various parts of the seed containing inhibitors, of which seed coat more content.

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