Isoflurane protects the isolated rat lungs against ischemia-reperfusion injury

摘要

Objective To study the effects of isoflurane on ischemia-reperfusion(IR)-induced injury in isolated rat lungs.Methods This study was performed on an isolated buffer-perfused rat lung model.There were four groups:CON group(n=6):perfusion for60min without ischemia;IR group(n=6):after perfusion for30min,then interruption of perfusion and ventilation for45min followed by reperfu-sion for30min;ISO1group(n=6):1.38%isoflurane was administered for30min before45min ischemia,then followed by30min reper-fusion;ISO2group(n=6):1.38%isoflurane was administered during the period of reperfusion.The Myeloperoxidase(MPO)activity of lung,neutrophils counts,tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD)activity,malondialdehyde(MDA)in perfusate,and the Wet-to-Dry lung weight ratios were measured.Results IR caused significant increases in the wet-to-dry lung weight ratios,the activity of lung MPO,the contents of MDA and TNF-αin perfusate,but decreasess in the SOD activity and neutrophils counts.Administration of isoflu-rane(both ISO1and ISO2groups)significantly protected against IR-induced injury the via inhibiting increases of MPO activity and contents of MDA and TNF-αin perfusate.Isoflurane significantly increased the SOD activity and neutrophils counts in perfusate.No difference was found between ISO1and ISO2groups.Conclusion Our results suggest that isoflurane protects the lungs against IR-induced injury in isolated rat lung model.