中国蛤蜊水提取物对小鼠急性肝损伤的保护作用

摘要

Objective： To explore the protective effect of water extract from Mactra chinensis muscle（WEMC） on CCl4-induced acute hepatic injury of mice.Methods： Mice were administered orally with the extract at the daily doses of 0.15,0.45 g/（kg·d）and 0.90 g/（kg·d）.Eight days later,6% CCl4 were intraperitoneally injected at the dose of 1 mL/kg.At 24 h after the injection,liver index and the contents of serum alanine aminotransferase（ALT）,total protein（TP）,albumin（Alb） and globulin（Glb） as well as A/G were investigated.On the other hand,the pathological changes in the hepatic tissues of each group were determined.Results： WEMC at each dose significantly lowered ALT in serum（P 0.01）.Meanwhile,the hepatic histology was also improved in three WEMC groups.Moreover,a significant increase in A/G was observed in each WEMC group when compared with the model group（P 0.01）.WEMC had a pronounced promoting effect on the recovery of mouse liver function but had little effect on liver index.Under a light microscope,it could be observed that WEMC had a marked effect on improving hepatic injury in mice.Conclusion： EMC can protect CCl4-induced liver damage of mice.%目的：研究中国蛤蜊水提取物（WEMC）对四氯化碳（CCl4）所致小鼠肝损伤的保护作用。方法：对小鼠经口灌胃0.15、0.45、0.90g/（kg.d）的中国蛤蜊水提取物,8d后,以1mL/kg的剂量一次性腹腔注射6%的CCl4,24h后测定小鼠的肝指数、血清谷丙转氨酶（ALT）、总蛋白（TP）、白蛋白（Alb）、球蛋白（Glb）、白球比（A/G）等指标,光镜下观察肝组织学变化。结果：中国蛤蜊水提取物各剂量组均能显著降低CCl4所致急性肝损伤小鼠的血清ALT的水平（P〈0.01）,各剂量组的A/G较模型组明显上升（P〈0.01）,且明显促进肝功能的恢复,但对肝指数无明显影响,光镜下可见中国蛤蜊水提取物可明显改善肝组织损伤程度。结论：中国蛤蜊水提取物对CCl4所致小鼠肝损伤有保护作用。