鲨鱼软骨多糖中硫酸角质素分离方法研究

摘要

The arm of this research was to explore a simple and effective method for the separation and detection of keratan sulfate(KS)in shark cartilage. The method to seperate KS from polysaccharide was ethanol precipita-tion, the uronic acid content was measured by sulfuric acid-carbazole,the KS content was measured by Mono Q HPLC.The precipitation condition was:the salt concentration was 2.0%, ethanol concentration was lower than 56%;the salt concentration was 1.5%, ethanol concentration was lower than 58%;the salt concentration was 1.0%, ethanol concentration was lower than 60%. The ethanol precipitation was a reliable industrial method to seperate KS from polysaccharide , Mono Q HPLC method was a simple and effective method to detect KS.%探索简单有效的分离和检测鲨鱼软骨多糖中硫酸角质素组分的方法。使用乙醇分级沉淀法分离多糖中硫酸角质素，使用硫酸-咔唑法测定糖醛酸含量，Mono Q HPLC法测定硫酸角质素含量。：硫酸角质素从多糖中分离的条件：氯化钠浓度为2.0%，乙醇浓度小于56%时；氯化钠浓度为1.5%，乙醇浓度小于58%时；氯化钠浓度为1.0%，乙醇浓度小于60%时。使用乙醇分级沉淀法可实现鲨鱼软骨多糖中硫酸角质素组分的分离，Mono Q HPLC法可简单快速检测多糖中硫酸角质素组分。