HPLC测定维拉佐酮片的含量

摘要

目的 建立高效液相色谱法(HPLC)测定维拉佐酮片含量的方法.方法 采用Waters Symmetry色谱柱(250 mm×4.6 mm,5μm),以0.01 mol/L醋酸铵溶液(取醋酸铵0.77 g,加水950 mL,加三乙胺1 mL,用醋酸调节pH值至6.0±0.05,加水至1000 mL)-乙腈(50:50)为流动相,流速1.0 mL/min,检测波长242 nm,进样体积20μL.结果 维拉佐酮峰面积与浓度在12.1~28.1μg/mL范围内线性良好(r=0.9999);回收率为99.88%(n=9),RSD为0.37%.结论 此法操作简便,结果准确度高,精密度好,可用于维拉佐酮片的含量测定.%Objective To establish an HPLC method for content determination of Vilazodone Tablets. Methods The Waters Symmetry C18 column (4.6 mm×250 mm, 5 μm) was adopted. The mobile phase consisted of acetonitrile-0.01 mol/L ammonium acetate solution (50:50). The flow rate was 1.0 mL/min. The detection wavelength was 242 nm and the injection volume was 20 μL. Results A good linear correlation with peak areas was observed within the range of 12.1-28.1 μg/mL for vilazodone (r=0.9999). The average recovery was 99.88 % (n=9), with RSD of 0.37 %. Conclusion The method is simple, accurate and can be used for the content determination of Vilazodone Tablets.